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American Society of Microbiology ABMM Sample Questions:

1. A patient presents with perianal itching, particularly at night. Microscopic examination of cellophane tape preparation from the perianal area reveals oval-shaped eggs with one flattened side. The MOST likely causative organism is:

A) Ascaris lumbricoides
B) Trichuris trichiura
C) Strongyloides stercoralis
D) Enterobius vermicularis

2. A patient presents with a pruritic rash consisting of raised, serpiginous tracks on their skin. The patient recently returned from a Caribbean vacation and reported walking barefoot on the beach. The MOST likely causative organism is:

A) Sarcoptes scabiei
B) Larvae of Ancylostoma braziliense
C) Larvae of Strongyloides stercoralis
D) Pediculus humanus

3. A patient with severe combined immunodeficiency (SCID) develops a progressive respiratory illness.
Bronchoalveolar lavage fluid is examined using direct fluorescent antibody (DFA) staining, which reveals numerous cysts of Pneumocystis jirovecii. The cysts appear:

A) Cup-shaped or helmet-shaped with internal structures.
B) Small and round with a thick wall, containing sporozoites.
C) Oval-shaped with a thin wall and internal nuclei.
D) Large and multinucleated with prominent endospores.

4. A clinical microbiology laboratory is validating a new point-of-care test for the rapid detection of influenza A and B viruses. To accurately determine the clinical sensitivity of the test, it should be compared to a gold standard method using:

A) A large number of prospectively collected clinical samples from patients with suspected influenza, with results confirmed by RT-PCR.
B) Spiked samples containing known concentrations of influenza antigens.
C) Samples from patients with other respiratory viral infections to assess cross-reactivity.
D) A panel of known positive and negative samples stored at room temperature.

5. A microbiology laboratory receives a cerebrospinal fluid (CSF) sample from a patient with suspected meningitis. Gram stain shows numerous Gram-negative diplococci. The organism grows on chocolate agar but not on blood agar without added CO2. The MOST likely identification is:

A) Streptococcus pneumoniae
B) Neisseria meningitidis
C) Listeria monocytogenes
D) Haemophilus influenzae

Solutions: