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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Flow cytometry employs a combination of fluorescent antibody tagging of cells and analysis with laser light scatter.
What principle(s) of flow cytometry are employed when performing immunophenotyping:

A) Fluorescent antibody tagging and light scatter
B) Defraction gradients and impedance
C) Impedance
D) Defraction gradients

2. Unconjugated bilirubin is a byproduct of RBC breakdown, or hemolysis. It would be expected to see an increase in unconjugated bilirubin when hemolysis is occuring at an increased rate. The liver enzymes would not remain at normal levels if there were a viral infection of the liver, chemical damage to the liver, or obstruction of bile ducts.
Chemistry
A patient presents with an elevation of unconjugated bilirubin, normal serum alkaline phosphatase, normal liver enzymes, and no bilirubin in the urine. This combination would suggest:

A) Chemical damage to the liver.
B) Increased rate of hemolysis
C) Obstruction of common bile duct
D) Viral infection of the liver.

3. Lipids tend to form a "maltese cross" pattern when viewed in polarized light, which helps to differentiate them from other urine microscopic components.
Substances found in urinary sediment that are more easily distinguished by use of polarized microscope are:

A) Crystals
B) Lipids
C) RBC
D) WBC
E) Casts

4. The proper pH for a unit of platelets must be above 6.2. According to the FDA, accurate pH measurement is time dependent, and samples should be tested within 1 hour of sampling, or as suggested by the manufacturer of the pH measurement system. It is recommended that a pH meter or gas analyzer be routinely used rather than pH (nitrazine) paper. However, if the laboratory chooses to determine pH measurements with nitrazine paper, the selected paper should read in increments of one-tenth units, or it may provide inaccurate measurements. Maintaining proper pH requires at least 35 ml, but preferably 50-70 ml of plasma per platelet unit.
In order to prevent a loss of viability in platelet concentrates during storage the pH must be maintained above:

A) 4.5
B) 7.5
C) 5.0
D) 6.2

5. Beer's law is based on the fact that absorbance is directly proportional to the concentration of a solution.
Therefore, stray light can alter the absorbance results in this type of assay.
Deviations from Beer's Law are caused by:

A) polychromatic light
B) very low concentration of absorbing material
C) stray light
D) very high concentrations of substance being measured in a colorimetric reaction

Solutions: